Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution

Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution

Blog Article

Abstract Highly sensitive airborne virus monitoring is critical for preventing and containing epidemics.However, the detection of airborne viruses at ultra-low concentrations remains challenging due to the lack of ultra-sensitive methods and easy-to-deployment equipment.Here, we present an integrated microfluidic cartridge that can accurately detect SARS-COV-2, Influenza A, B, and respiratory syncytial virus with a sensitivity of 10 copies/mL.When integrated with a high-flow aerosol sampler, our microdevice can achieve a sub-single-copy spatial resolution of 0.

83 copies/m3 for airborne virus surveillance with an air flow rate of cent dyyni 400 L/min and a sampling time of 30 minutes.We then designed a series of virus-in-aerosols monitoring systems (RIAMs), including versions of a multi-site sampling RIAMs (M-RIAMs), a stationary real-time RIAMs (S-RIAMs), and a roaming real-time RIAMs (R-RIAMs) for different application scenarios.Using M-RIAMs, we performed a comprehensive 1073spx evaluation of 210 environmental samples from COVID-19 patient wards, including 30 aerosol samples.The highest positive detection rate of aerosol samples (60%) proved the aerosol-based SARS-CoV-2 monitoring represents an effective method for spatial risk assessment.

The detection of 78 aerosol samples in real-world settings via S-RIAMs confirmed its reliability for ultra-sensitive and continuous airborne virus monitoring.Therefore, RIAMs shows the potential as an effective solution for mitigating the risk of airborne virus transmission.